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Piero R. Bianco
B.S., Biology, 1987 -
Abilene Christian University
Ph. D., Biochemistry and Molecular Biology, 1993 - University of Texas Medical School
at Houston Postdoctoral Scholar, 1994-2000
- University of California, Davis, CA |
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Current address:
Associate Professor
Department of Microbiology and Immunology
Center for Single Molecule Biophysics
321 Cary Hall
University at Buffalo
The State University of New York
Buffalo, NY 14214
Office Tel: (716) 829-2599
Fax (716) 829-2158
email: pbianco@buffalo.edu |
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Bianco, PR and Weinstock, GM (1994).
Automated determination of β-galactosidase specific activity.
Biotechniques 17:974-80. PMID: 7840979.
Bianco, PR and Weinstock, GM (1996).
Interaction of the RecA protein of Escherichia coli with single-stranded
oligodeoxyribonucleotides.
Nucleic Acids Res. 24:4933-9. PMID: 9016663.
Bianco, P.R. and Kowalczykowski, S.C. (1997).
The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as
the single strand of DNA containing the sequence 5'-GCTGGTGG-3'.
Proc. Natl. Acad. Sci. USA, 94, 6706-6711.
PMID: 9192629; PMCID: PMC21222.
[Abstract]
[Full
Text] [PDF]
Bianco, PR and Weinstock, GM (1998).
Characterization of RecA1332 in vivo and in vitro. A role for α-helix E as a
liaison between the subunit-subunit interface and the DNA and ATP binding
domains of RecA protein.
Genes to Cells 3:79-97. PMID: 9605403.
Bianco, PR and Weinstock, GM (1998).
UTP is a cofactor for the DNA strand exchange reaction performed by the RecA
protein of Escherichia coli.
Biochemistry 37:7313-20. PMID: 9585545.
Arnold, D.A., Bianco, P.B., and
Kowalczykowski, S.C. (1998).
The Reduced Levels of χ
Recognition Exhibited by the RecBC1004D Enzyme Reflect Its
Recombination Defect in Vivo.
J. Biol. Chem., 273, 16476-16486. PMID:
9632715. [Abstract] [Full
Text]
[PDF]
Bianco, P.R., Tracy, R.B., and
Kowalczykowski, S.C. (1998).
DNA strand exchange proteins: A biochemical and physical comparison.
Front. Biosci., 3, D570-D603.
PMID: 9632377. [Abstract] [Full Text] [PDF] Bianco, P.R. and
Kowalczykowski, S.C. (1999).
RecA protein.
In: Encyclopedia of Life Sciences, Nature
Publishing Group, London, http://www.els.net
[Abstract] [Full
Text]
[PDF]
[Preprint.PDF]
Bianco, P.R. and Kowalczykowski, S.C.
(2000).
The translocation step size and mechanism of the RecBC helicase.
Nature,
405,
368-372. PMID: 10830968.
[Abstract] [Full
Text] [PDF]
(see Dispatch in Current Biology:
[Abstract]
[Full Text]
[PDF])
(see News &
Information
at UC Davis News Service
[Full Text]
[Printable Version])
(see Science
Blog [PDF])
Bianco, P.R., Brewer, L.R., Corzett, M., Balhorn,
R., Yeh,
Y., Kowalczykowski, S.C., and
Baskin, R.J. (2001).
Processive translocation
and DNA unwinding by individual RecBCD enzyme molecules.
Nature, 409,
374-378. PMID: 11201750.
[Abstract]
[Full
Text]
[PDF]
Movies!
37°C:
1
mM ATP
23°C:
1 mM ATP;
250
µM ATP #1;
250
µM ATP #2;
no ATP
(see
coverage in Biophotonics (2001) 8, 48-49
[TOC]
[PDF]
(see UC Davis Division of Biological Sciences Newsletter [PDF])
Press coverage:
Capitol
Public Radio
- January 17, 2001 - Full transcript
San Francisco
Chronicle - January 20, 2001 - excerpt
Full
article - "Watch DNA Unzip in Micromovie-Molecular action film highlights potential of
nanotechnology"
San Francisco
Chronicle - January 24, 2001 - excerpt
Full article: "A Little Spark Of Inspiration"
San Francisco Chronicle -
January 26, 2001 - excerpt
Full article: "Never Metatag We Didn't Like"
News &
Information
at UC Davis News Service
[Full Text]
Nanotech Dreams -
The
Scientist
16[5]:34, Mar.
4, 2002
- Excerpt
Full article
Davis Community
Television -
February 8, 2001
7:00 PM - "Get The Word Out." Double helix unwinds on camera. Stephen
Kowalczykowski interviewed by Jon Price. (Rerun on Tues Feb 13, 2001 at 7:30
PM). [VIDEO]
Spies, M.,
Bianco, P.R., Dillingham, M.S.,
Handa, N.,
Baskin, R.J.,
and
Kowalczykowski, S.C.
(2003)
A molecular throttle: The recombination hotspot
c
controls DNA translocation by the RecBCD helicase.
Cell, 114, 647-654. PMID:
13678587.
[Abstract]
[Full
Text] [PDF]
[Featured Article]
[Supplemental Table S1]
[Supplemental Table S2]
[Supplemental Table S3]
[Supplemental Movie S1]
[Supplemental Movie S2]
[Supplemental Movie S3]
(see
News & Features at
Bio.com
[Full Text]
[PDF])
(see News &
Information
at UC Davis News Service
[Full Text]
[Printable Version])
(see
News &
Information
at UC Davis News Service
[Full Text]
[Printable Version])
Handa, N.,
Bianco, P.R.,
Baskin, R.J.,
and
Kowalczykowski, S.C.
(2005)
Direct visualization of RecBCD movement reveals co-translocation of the RecD
motor after c-recognition.
Mol. Cell,
17,
745-750. PMID: 15749023.
[Abstract]
[Full
Text]
[PDF] [Supplemental
Data] [Movie 1
- Chi0] [Movie 2 - Chi+]
Bianco, P.R. and
Kowalczykowski, S.C.
(2005).
RecA protein.
In: “Encyclopedia of Life Sciences”, John
Wiley & Sons, Ltd: Chichester,
http://www.els.net/ [doi:10.1038/npg.els.0003925].
[Abstract] [PDF]
[HTML]
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