Dillingham, Mark

 

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Mark Dillingham

B.Sc. (Hons.), 1996 - 1st in Biochemistry - University of Bristol

D. Phil. "Biochemical studies on DNA helicases", 1999 - Lincoln College, Oxford

Postdoctoral Fellow, 2001-2002 - University of California, Davis, CA
  Current address:

Senior Lecturer
University of Bristol
School of Biochemistry
Medical Sciences Building
University Walk
Clifton, Bristol BS8 1TD
Tel: (0117) 3312159
Mark.Dillingham@bristol.ac.uk

Szczelkun, M.D., Dillingham, M. S., Janscak, P., Firman, K. and Halford,S.E. (1996).
Repercussions of DNA tracking by the type IC restriction-endonuclease EcoR124I on linear, circular and catenated substrates.
EMBO J., 15, 6335-6347. PMID: 8947056; PMCID: PMC452456.

Soultanas, P., Dillingham, M. S. and Wigley, D. B. (1998).
Escherichia coli ribosomal protein L3 stimulates the helicase activity of the Bacillus stearothermophilus PcrA helicase.
Nucl. Acids Res. 26, 2374-2379. PMID: 9580688; PMCID: PMC147557.

Velankar, S.S., Soultanas, P., Dillingham, M. S., Subramanya, H.S. and Wigley, D. B. (1999).
Crystal structures of complexes of PcrA helicase with a DNA substrate indicate an inchworm mechanism.
Cell 97, 75-84. PMID: 10199404.

Soultanas, P., Dillingham, M. S., Velankar, S.S. and Wigley, D. B. (1999).
DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase.
J. Mol. Biol. 290, 137-148. PMID: 10388562.

Soultanas, P., Dillingham, M. S., Papadopoulos, F., Phillips, S.E.V., Thomas, C.D. and Wigley, D. B. (1999).
Plasmid replication initiator protein RepD increases the processivity of PcrA DNA helicase.
Nucl. Acids Res. 27, 1421-1428. PMID: 10037801; PMCID: PMC148333.

Dillingham, M. S., Soultanas, P. and Wigley, D. B. (1999).
Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation.
Nucl. Acids Res. 27, 3310-3317. PMID: 10454638; PMCID: PMC148564.

Dillingham, M. S., Wigley, D. B. and Webb, M.R. (2000).
Demonstration of unidirectional single stranded DNA translocation by PcrA helicase : measurement of step size and translocation speed.
Biochemistry 39, 205-212. PMID: 10625495.

Soultanas, P., Dillingham, M. S., Wiley, P., Webb, M.R. and Wigley, D. B. (2000).
Uncoupling DNA translocation and helicase activity in PcrA : direct evidence for an active mechanism.
EMBO J. 19, 3799-3810. PMID: 10899133; PMCID: PMC313991.

Dillingham, M. S., Soultanas, P., Wiley, P., Webb, M.R. and Wigley, D. B. (2001).
Defining the roles of individual residues in the single-stranded DNA binding site of PcrA helicase.
Proc. Natl. Acad. Sci. (USA) 98, 8381-8387. PMID: 11459979; PMCID: PMC37447.

Dillingham, M. S. and Kowalczykowski, S.C.  (2001).
A Step Backward in Advancing DNA Replication: Rescue of Stalled Replication Forks by RecG. 
Mol. Cell, 8, 734-736. PMID: 11684009. [Abstract] [Full Text] [PDF]

Dillingham, M. S., Wigley, D. B. and Webb, M.R. (2002)
Direct measurement of single stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine.
Biochemistry 41, 643-651. PMID: 11781105.

Spies, M., Dillingham, M.S., and Kowalczykowski, S.C. (2003).
DNA helicases.
In “McGraw-Hill Yearbook of Science & Technology”, New York, N. Y., 95-98 [PDF] [scanned-PDF]  [Page proof]

Dillingham, M.S., Spies, M., and Kowalczykowski, S.C. (2003)
RecBCD enzyme is a bipolar DNA helicase.
Nature, 423, 893 - 897. PMID: 12815438. [Abstract] [Full Text] [PDF] [Supplementary Figure] [Supplementary Figure Legend and Methods]
(see News & Views in Nature Structural Biology 10, 499 - 500 (2003)  [Full Text]  [PDF])
(see Highlight in Nature Reviews Molecular Cell Biology 4, 513 (2003); doi:10.1038/nrm1162. [Full Text] [PDF])
(Must Read paper by Faculty of 1000 [Full Text])
(see News & Information at UC Davis News Service  [Full Text] [Printable Version])
 Access the recommendation on F1000Prime

Spies, M., Bianco, P.R., Dillingham, M.S., Handa, N., Baskin, R.J., and Kowalczykowski, S.C.  (2003)
A molecular throttle: The recombination hotspot c controls DNA translocation by the RecBCD helicase.
Cell, 114, 647-654. PMID: 13678587. [Abstract] [Full Text] [PDF] [Featured Article] [Supplemental Table S1] [Supplemental Table S2] [Supplemental Table S3] [Supplemental Movie S1] [Supplemental Movie S2] [Supplemental Movie S3]
(see News & Features at Bio.com  [Full Text] [PDF])
(see News & Information at UC Davis News Service  [Full Text] [Printable Version])
(see News & Information at UC Davis News Service  [Full Text] [Printable Version])

Singleton, M. R., Dillingham, M.S., Gaudier, M., Kowalczykowski, S.C., and Wigley, D. B. (2004)
Crystal structure of RecBCD reveals a machine for processing DNA breaks.
Nature, 432, 187-193. PMID: 15538360.  [Abstract] [Full Text] [PDF]
(see News & Views  [Full Text] [PDF])
(see Nature "Highlights"  [Full Text])
 Access the recommendation on F1000Prime

Spies, M., Dillingham, M.S., and Kowalczykowski, S.C. (2005)
DNA helicases.
In “McGraw-Hill Encyclopedia of Science & Technology”, New York, N. Y. [Preprint]

Dillingham, M.S., Webb, M.R., and Kowalczykowski, S.C. (2005)
Bipolar DNA translocation contributes to highly processive DNA unwinding by RecBCD enzyme.
J. Biol. Chem., 280, 37069 - 37077 (published online July 22, 2005 as 10.1074/jbc.M505520200). PMID: 16041061. [Abstract] [Full Text] [PDF] [Supplemental Data]

Spies, M., Dillingham, M.S., and Kowalczykowski, S.C. (2005).
Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.
 J. Biol. Chem., 280, 37078 – 37087 (published online July 22, 2005 as 10.1074/jbc.M505520200). PMID: 16041060. [Abstract] [Full Text] [PDF]

Chédin, F., Handa, N., Dillingham, M.S., and Kowalczykowski, S.C. (2006).
The AddAB helicase/nuclease forms a stable complex with its cognate chi sequence during translocation.
J. Biol. Chem., 281, 18610-18617. PMID: 16632468. (published online April 21, 2006 as doi:10.1074/jbc.M600882200).  [Abstract] [Full Text] [PDF]

Dillingham, M.S., Tibbles, K.L., Hunter, J.L., Bell, J.C., Kowalczykowski, S.C., and Webb, M.R. (2008).
Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.
Biophys. J., 95, 3330-3339 (published online July 3, 2008, as 10.1529/biophysj.108.133512). PMID: 18599625; PMCID: PMC2547451 [Abstract] [Full Text] [PDF]

Dillingham, M.S. and Kowalczykowski, S.C. (2008).
 RecBCD enzyme and the repair of double-stranded DNA breaks.
Microbiol. Mol. Biol. Rev., 72, 642–671 (published online as doi:10.1128/MMBR.00020-08). PMID: 19052323; PMCID: PMC2593567 [Abstract] [Full Text] [PDF]

Handa, N., Yang, L., Dillingham, M. S., Kobayashi, I., Wigley, D.B., and Kowalczykowski, S.C. (2012)
Molecular determinants responsible for recognition of the single-stranded DNA regulatory sequence, χ, by RecBCD enzyme.
 Proc. Natl. Acad. Sci. U.S.A., 109, 8901-8906 (published online 17 May 2012, 10.1073/pnas.1206076109). PMID: 22603794; PMCID: PMC3384139 [Abstract] [Full Text] [PDF] [Supporting Information]

Yang, L., Handa, N., Liu, B., Dillingham, M. S., Wigley, D.B., and Kowalczykowski, S.C. (2012)
Alteration of χ recognition by RecBCD reveals a regulated molecular latch and suggests a channel-bypass mechanism for biological control.
Proc. Natl. Acad. Sci. U.S.A., 109, 8907-8912 (published online 17 May 2012, 10.1073/pnas.1206081109). PMID: 22603793; PMCID: PMC3384165  [Abstract] [Full Text] [PDF] [Supporting Information]