2010

 

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Amitani, I., Liu, B., Dombrowski, C.C., Baskin, R.J., and Kowalczykowski, S.C. (2010)
Watching individual proteins acting on single molecules of DNA.
In:
Single Molecule Tools: Fluorescence Based Approaches, Part A, Methods Enzymol., 472, Nils G. Walters, editor, Burlington: Academic Press, 261-291
ISBN: 978-0-12-374954-3; doi: 10.1016/S0076-6879(10)72007-3PMID: 20580968; PMCID: PMC3119597  [Abstract] [Full Text] [PDF]

Hilario, J. and Kowalczykowski, S.C. (2010)
Visualizing protein-DNA interactions at the single-molecule level.

Curr. Opin. Chem. Biol.,
14, 15-22. PMID: 19945909; PMCID: PMC2819561 [Abstract] [Full Text] [PDF]

Kemp, M.G., Mason, A.C., Carreira, A., Reardon, J.T., Haring, S.J., Borgstahl, G.E.O., Kowalczykowski, S.C., Sancar, A., and Wold, M.S. (2010)
An alternative form of replication protein A expressed in normal human tissues supports DNA repair.
J. Biol. Chem., 285,
4788-4797 (published online 7 December 2009, as doi:10.1074/jbc.M109.079418). PMID: 19996105;
PMCID: PMC2836084 [Abstract] [Full Text] [PDF]

Cejka, P. and Kowalczykowski, S.C. (2010)
The full-length Saccharomyces cerevisiae Sgs1 protein is a vigorous DNA helicase that preferentially unwinds Holliday junctions.
J. Biol. Chem., 285,
8290-8301 (published online January 19, 2010, as doi:10.1074/jbc.M109.083196).  PMID: 20086270;
PMCID: PMC2832980  [Abstract] [Full Text] [PDF]

Forget. A.L. and Kowalczykowski, S.C. (2010)
Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.
Trends in Cell Biology,
20, 269-276 (published online March 17, 2010, as doi:10.1016/j.tcb.2010.02.004). PMID: 20299221;
PMCID: PMC2862779 [Abstract] [Full Text] [PDF]
(see Trends in Cell Biology - Most Read Articles [PDF])

Cejka, P., Cannavo, E., Polaczek, P., Masuda-Sasa, T., Pokharel, S., Campbell, J.L., and Kowalczykowski, S.C. (2010)
DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2.
Nature, 467, 112-116 (doi: 10.1038/nature09355). PMID: 20811461; PMCID: PMC3089589  [Abstract] [Full Text] [PDF] [Supplementary Information]

(see Egghead blog about research at UC Davis: DNA repair machine on the bench [PDF])

Jensen, R.B., Carreira, A., and Kowalczykowski, S.C. (2010)
Purified human BRCA2 stimulates RAD51-mediated recombination.
Nature,
467, 678-683 (published online: August 22, 2010, doi: 10.1038/nature09399). PMID: 20729832; PMCID: PMC2952063  [Abstract] [Full Text] [PDF] [Supplementary Information]

(see UC Davis interview: [Video-Flash] [Video-MPEG4])
(see Nature News & Views: DNA Repair: A protein giant in its entirety [PDF])
(see Nature News: Breast cancer protein is finally purified Latest News [PDF])
(see American Cancer Society News: New finding may unlock secrets of BRCA mutations [PDF])
(see Biocompare, Life Sciences Articles, News: Protein made by breast cancer gene purified [PDF])
(see Biomedical Beat - National Institute of General Medical Sciences: BRCA2 Breakthrough  News [PDF])
(see Bionity.com: Protein made by breast cancer gene purified [PDF])
(see BioTechniques: New gels shed light on cancer-related protein [PDF])
(see Congressionally Directed Medical Research Programs (CDMRP), Breast Cancer Research Highlights: Successful isolation of BRCA2 (breast cancer type 2 susceptibility protein) and elucidation of its mechanistic functions underlying breast cancer tumorigenesis [PDF])
(see Davis Enterprise: Scientists dig into breast cancer origins [PDF])
(see Deutsches Ärzteblatt:
Brustkrebs-Protein im Labor hergestellt [PDF])
(see e! Science News: Protein made by breast cancer gene purified [PDF])
(see ebiotrade: "Nature": successfully isolated and purified human lung cancer gene BRCA2 protein [PDF])
(see Eurekalert.org: Protein made by breast cancer gene purified [PDF])
(see GENNewsHighlights: Researchers succeed in purifying protein expressed by BRCA2 [PDF])
(see GenomeWeb: The elusive protein, found [PDF])
(see Guttenberg Municipal Hospital, HealthDay Daily Newsfeed: Inner workings of gene tied to breast, ovarian cancer revealed [PDF])
(see Higher Colleges of Technology, HCT Libraries: BRCA2, purified, reveals some of its secrets [PDF])
(see The Hindu: Protein made by breast cancer gene 'purified' [PDF])
(see Innovations Report: Protein made by breast cancer gene purified [PDF])
(see Journal of the National Cancer Institute: BRCA2, purified, reveals some of its secrets [PDF])
(see Justmeans: New finding may unlock secrets of BRCA mutations [PDF])
(see Lahey Hospital and Medical Center, HealthDay News: Inner workings of gene tied to breast, ovarian cancer revealed [PDF])
(see Mammary Cell News: Breast cancer protein is finally purified [PDF])
(see MedGuru: Pure form of tumor protein extracted successfully [PDF])
(see Medical News Today: Purification of protein made by breast cancer gene [PDF])
(see medwireNews: Purification of BRCA2 protein allows understanding of its role in DNA repair [PDF])
(see ModernMedicine: Researchers isolate BRCA2 protein for first time [PDF])
(see MSN Health and Fitness: Inner workings of
gene tied to breast, ovarian cancer revealed [PDF])
(see Natureasia.com: BRCA2 protein characterized [PDF])
(see News, Chauthi Duniya: Breast-cancer protein purified [PDF])
(see NewScientist Health: Fundamental breast and ovarian cancer protein isolated [PDF])
(see Pharmaceutical Online: Protein made by breast cancer gene purified [PDF])
(see Santa Maria Law: Breast cancer research [PDF])
(see Science Codex: Protein made by breast cancer gene purified [PDF])
(see ScienceDaily: Protein made by breast cancer gene purified [PDF])
(see Softpedia News: Breast cancer protein scrutinized [PDF])
(see Synthesis, Fall/Winter 2010: Protein made by breast cancer gene purified [PDF])
(see Synthesis, Spring/Summer 2011: Solving a genetic puzzle: Major findings open doors in DNA repair research [PDF])
(see USNews Health: Inner workings of gene tied to breast, ovarian cancer revealed [PDF])
(see UC Davis Health System News: Protein made by breast cancer gene purified [PDF])
(see UC Davis News & Information: Protein made by breast cancer gene purified [PDF] [printable])
(see WSFA 12 News Montgomery, AL: Inner workings of gene tied to breast, ovarian cancer revealed [PDF])
(see Yahoo! Movies India: Breast-cancer protein purified [PDF])

Cejka, P., Plank, J.L., Bachrati, C.Z., Hickson, I.D., and Kowalczykowski, S.C. (2010)
Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3.
Nature Struct. Mol. Biol.,
17, 1377-1382 (published online October 10, 2010; doi: 10.1038/nsmb.1919). PMID: 20935631; PMCID: PMC2988882  [Abstract] [Full Text] [PDF] [Supplementary Material]

(see California Aggie: DNA separates like a magician's rings [PDF])
(see CheckOrphan, Research News: DNA and the 'magic rings' trick [PDF])
(see Davis Enterprise: Study shows DNA acts like a 'magic rings' trick [PDF])

(see Healthcanal: DNA and the 'magic rings' trick [PDF])
(see PhysOrg.com news: DNA and the 'magic rings' trick [PDF])
(see UC Davis Comprehensive Cancer Center: DNA and the 'magic rings' trick [PDF])
(see UC Davis Health System: DNA and the 'magic rings' trick [PDF])
(see UC Davis News and Information: DNA and the 'magic rings' trick  [Printable] [PDF])
(see UC Newsroom: Genetic magic trick may hold clues to Bloom's syndrome [PDF])