Faculty of 1000 | Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament.

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Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament.
Alexeev A, Mazin A, Kowalczykowski SC
Nat Struct Biol 2003 Mar 10(3):182-6 [abstract on PubMed] [related articles] [order article]

Selected by | Peter Becker / Dale Ramsden
First evaluation 4 Apr 2003 | Latest evaluation 7 May 2003

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Faculty Comments

Faculty Member	Comments
Peter Becker Adolf-Butenandt-Institut, Germany DEVELOPMENTAL BIOLOGY New Finding	Sometimes one wonders how complex DNA recombination processes can occur with DNA wrapped up in nucleosomes. This paper shows that one of the protagonists of yeast recombinational repair, Rad54, is a remodeling ATPase able to induce sliding of nucleosomes on DNA. The sliding activity is stimulated by contact with a Rad51-ssDNA complex. Rad54 may, therefore, move nucleosomes during the search for sequences homologous to the DNA break. This is a nice example of how nucleosome remodeling is integrated into a more complex process. Evaluated 7 May 2003 cite this evaluation
Dale Ramsden University of North Carolina, United States STRUCTURAL BIOLOGY New Finding	This paper provides the first clear argument that the homologous recombination co-factor rad54 can restructure chromatin. The authors determine that Rad54 alone is sufficient to remodel mononucleosomes, but this activity is strongly stimulated by the additional presence of a Rad51 nucleoprotein filament. Together with other recent reports, a picture is emerging of a complex multi-protein machine well designed for recombination in the context of natural chromosomes. Evaluated 4 Apr 2003 cite this evaluation

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How to cite the Faculty of 1000 evaluation(s) for this paper

1) To cite all the evaluations for this article:

Faculty of 1000: evaluations for Alexeev A et al Nat Struct Biol 2003 Mar 10 (3) :182-6 http://www.f1000biology.com/article/12577053/evaluation

2) To cite an evaluation by a specific Faculty member:

Peter Becker: Faculty of 1000, 7 May 2003 http://www.f1000biology.com/article/12577053/evaluation

Dale Ramsden: Faculty of 1000, 4 Apr 2003 http://www.f1000biology.com/article/12577053/evaluation

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